Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers.
نویسندگان
چکیده
A simple and precise genotyping system based on PCR using type-specific primers was developed for the determination of genotypes A through F of hepatitis B virus (HBV). This assay system is considered to be a useful tool for the molecular diagnosis of HBV infection and for large-scale surveys.
منابع مشابه
Hepatitis C virus infection and genotypes in blood donors
Abstract: Genotyping of the hepatitis C virus (HCV) is important for designing therapeutic strategies and regional specific diagnostic assays. The aim of this study was to identify the HCV genotypes in HCV infected blood donors. This is the first report on HCV genotypes in blood donors in Iran. In this cross-sectional study, 103 blood donors with hepatitis C were investigated for HCV genotype...
متن کاملHepatitis B virus genotyping in asymptomatic HBV blood donors in Tehran
Abstract Background and Objectives Hepatitis B virus (HBV) infection remains a serious global health challenge. Hepatitis B infection is one of the major diseases transmitted by blood transfusion. Infection with the virus can cause extensive liver damage. So far, 10 genotypes have been identified for hepatitis B. The aim of this study was to do Hepatitis B virus genotyping in asymptomatic HBV...
متن کاملThe Relationship between Hepatitis C Genotypes and Viral Load: a study on hepatitis C patients in Kerman, Iran using Real-time PCR
Abstract Background: Distribution of hepatitis C virus genotypes varies geographically and can be associated with clinical aspects. The virus has six major genotypes and eleven sub-genotypes. According to the recent studies, in hepatitis C patients, treatment duration and ribavirin dosage should be determined based on the type of virus genotype. Be...
متن کاملDetection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR
Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...
متن کاملThe Frequency of Genotype D of Hepatitis B Virus in Isfahan, Iran
Background: Approximately 600,000 deaths occur every year as a result of the acute and chronic consequences of hepatitis B virus infection. Ten different hepatitis B virus genotypes have been identified with distinct geographical distributions. Different clinical outcomes, including the rate of mutations, development of hepatocellular carcinoma, chronicity, response to treatment, transplantatio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 39 1 شماره
صفحات -
تاریخ انتشار 2001